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PYK2 (phospho Tyr579) Antibody

Product Specifications

Background

Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.

NCBI Gene ID

2185

Swiss Prot

Q14289

Accession Number

NP_004094.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PYK2 (phospho Tyr579) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 579 (E-D-Y (p) -Y-K) derived from Human PYK2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

116 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

PTK2B

NCBI Official Name

Protein tyrosine kinase 2 beta

NCBI Organism

Homo sapiens

Background Reference 01

Lev S., Nature 376:737-745 (1995) .

Background Reference 02

Herzog H., Genomics 32:484-486 (1996) .

Background Reference 03

Sasaki H., J. Biol. Chem. 270:21206-21219 (1995) .

Other Product Names

CADTK, FADK 2, FAK2, PTK2B, RAFTK

Tested Applications

WB, IHC

Protein ID

27886584

Physical Properties

Liquid

Curated Selection

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