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Connexin 43 (phospho Ser265) Antibody

Product Specifications

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

NCBI Gene ID

2697

Swiss Prot

P17302

Accession Number

NP_000156.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Connexin 43 (phospho Ser265) antibody was raised against a peptide sequence around phosphorylation site of Serine 265 (Q-K-Y (p) -A-Y) derived from Human Connexin 43.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Fragment

IgG

Specificity

Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Serine 265

Applications Notes

Western Blot: 1:500~1:1000

Symbol

GJA1

NCBI Official Name

Gap junction protein, alpha 1, 43kDa

NCBI Organism

Homo sapiens

Background Reference 01

Toshihiko Toyofuku, J. Biol. Chem., May 1998; 273: 12725.

Background Reference 02

Toshihiko Toyofuku, J. Biol. Chem., Jan 1998; 273: 1519.

Background Reference 03

Masayuki Shimada, Biol Reprod, Apr 2001; 64: 1255.

Background Reference 04

Merry L. Lindsey, Circulation, Jun 2006; 113: 2919 - 2928.

Other Product Names

CX43, CXA1, CXN-43, GJA1

Tested Applications

WB

Protein ID

4504001

Physical Properties

Liquid

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