FOS (phospho Thr232) Antibody

Product Specifications

Background

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

NCBI Gene ID

2353

Swiss Prot

P01100

Accession Number

NP_005243.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

FOS (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (V-A-T (p) -P-E) derived from Human FOS .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

FOS

NCBI Official Name

FBJ murine osteosarcoma viral oncogene homolog

NCBI Organism

Homo sapiens

Background Reference 01

Van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187 (1983) .

Background Reference 02

Hai T., Genes Dev. 3:2083-2090 (1989) .

Background Reference 03

Heilig R., Nature 421:601-607 (2003) .

Other Product Names

FOS, G0S7, Cellular oncogene fos

Tested Applications

WB, IHC

Protein ID

4885241

Physical Properties

Liquid

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