TIE2 (phospho Tyr992) Antibody

Product Specifications

Background

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

NCBI Gene ID

7010

Swiss Prot

Q02763

Accession Number

NP_000450.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

TIE2 (phospho Tyr992) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y (p) -V-K) derived from Human TIE2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

126 kDa

Fragment

IgG

Specificity

The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

TEK

NCBI Official Name

TEK tyrosine kinase, endothelial

NCBI Organism

Homo sapiens

Background Reference 01

Schooley K.A., Baum P.R. Oncogene 8:663-670 (1993)

Background Reference 02

Dunham I. Nature 429:369-374 (2004)

Background Reference 03

Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

Other Product Names

Angiopoietin-1 receptor, EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2