MAP2K3 (phospho Thr222) Antibody

Product Specifications

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

NCBI Gene ID

5606

Swiss Prot

P46734

Accession Number

NP_659731.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p) -M-D) derived from Human MAP2K3 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

39 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAP2K3

NCBI Official Name

Mitogen-activated protein kinase kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Derijard B., Science 267:682-685 (1995) .

Background Reference 02

Moriguchi T., J. Biol. Chem. 271:26981-26988 (1996) .

Background Reference 03

The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Other Product Names

MAP2K3, MAPKK 3, MEK3, MP2K3, PRKMK3

Tested Applications

WB, IHC

Protein ID

24638466

Physical Properties

Liquid

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