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MAP3K1 (phospho Thr1402) Antibody

Product Specifications

Background

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

NCBI Gene ID

4214

Swiss Prot

Q13233

Accession Number

NP_005912.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MAP3K1 (phospho Thr1402) antibody was raised against a peptide sequence around phosphorylation site of threonine 1402 (T-G-A (p) -G-F) derived from Human MAP3K1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

130 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAP3K1

NCBI Official Name

Mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

NCBI Organism

Homo sapiens

Background Reference 01

Schmutz J., Nature 431:268-274 (2004) .

Background Reference 02

Xia Y., Genes Dev. 12:3369-3381 (1998) .

Background Reference 03

Vinik B.S., Mamm. Genome 6:782-783 (1995)

Other Product Names

M3K1, MAP3K1, MAPKKK1, MEKK1

Tested Applications

WB, IHC

Protein ID

153945765

Physical Properties

Liquid

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