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CK-1alpha (phospho Tyr294) Antibody

Product Specifications

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

NCBI Gene ID

1452;122011

Swiss Prot

P48729; Q8N752

Accession Number

NP_001883.4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CK-1alpha (phospho Tyr294) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p) -T-F) derived from Human CK-1alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

37 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of CK-1alpha only when phosphorylated at tyrosine 294.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CSNK1A1, CSNK1A1L

NCBI Official Name

Casein kinase 1, alpha 1, casein kinase 1, alpha 1-like

NCBI Organism

Homo sapiens

Background Reference 01

Tapia C., FEBS Lett. 349:307-312 (1994) .

Background Reference 02

Fish K.J., J. Biol. Chem. 270:14875-14883 (1995) .

Background Reference 03

Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

Other Product Names

KC1A, CKI-alpha, CSNK1A1, alpha isoform, Casein kinase I

Tested Applications

WB, IHC

Protein ID

68303575

Physical Properties

Liquid

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