ABL1 (phospho Thr735) Antibody

Product Specifications

Background

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

NCBI Gene ID

Swiss Prot

P00519

Accession Number

NP_005148.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ABL1 (phospho Thr735) antibody was raised against a peptide sequence around phosphorylation site of threonine735 (S-V-T (p) -L-P) derived from Human ABL1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

135 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

ABL1

NCBI Official Name

ABL proto-oncogene 1, non-receptor tyrosine kinase

NCBI Organism

Homo sapiens

Background Reference 01

Fainstein E., Oncogene 4:1477-1481 (1989) .

Background Reference 02

Shtivelman E., Cell 47:277-284 (1986) .

Background Reference 03

Chissoe S.L., Genomics 27:67-82 (1995) .

Other Product Names

Abelson murine leukemia viral oncogene 1, ABL1, c-ABL, JTK7, p150

Tested Applications

Protein ID

62362414

Physical Properties

Liquid

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