HSF1 (phospho Thr142) Antibody

Product Specifications

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

NCBI Gene ID

3297

Swiss Prot

Q00613

Accession Number

NP_005517.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

HSF1 (phospho Thr142) antibody was raised against a peptide sequence around phosphorylation site of threonine 142 (L-L-T (p) -D-V) derived from Human HSF1 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

57 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.

Symbol

HSF1

NCBI Official Name

Heat shock transcription factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910 (1991) .

Background Reference 02

The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Background Reference 03

Schuetz T.J., Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915 (1991)

Other Product Names

HSF 1, HSTF1, Heat shock factor protein 1

Tested Applications

IHC

Protein ID

5031767

Physical Properties

Liquid

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