ENOS (phospho Thr494) Antibody

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

NCBI Gene ID

4846

Swiss Prot

P29474

Accession Number

NP_000594.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ENOS (phospho Thr494) antibody was raised against a peptide sequence around phosphorylation site of threonine 494 (R-K-K (p) -T-F derived from Human eNOS .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

140 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

NOS3

NCBI Official Name

Nitric oxide synthase 3 (endothelial cell)

NCBI Organism

Homo sapiens

Background Reference 01

Janssens S.P., J. Biol. Chem. 267:14519-14522 (1992) .

Background Reference 02

Janssens S.P., J. Biol. Chem. 267:22694-22694 (1992) .

Background Reference 03

Marsden P.A., FEBS Lett. 307:287-293 (1992)

Other Product Names

CNOS, EC-NOS, ECNOS, NOS, NOS3

Tested Applications

Protein ID

40254422

Physical Properties

Liquid

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