SNAI1 (phospho Ser246) Antibody

Product Specifications

Background

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA) -induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

NCBI Gene ID

6615

Swiss Prot

O95863

Accession Number

NP_005976.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

SNAI1 (phospho Ser246) antibody was raised against a peptide sequence around phosphorylation site of Serine 246 (T-F-S (p) -R-M) derived from Human SNAI1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

29 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of SNAI1 only when phosphorylated at serine 246.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

SNAI1

NCBI Official Name

Snail family zinc finger 1

NCBI Organism

Homo sapiens

Background Reference 01

Okubo T., Cancer Res. 61:1338-1346 (2001) .

Background Reference 02

Twigg S.R., Hum. Genet. 105:320-326 (1999) .

Background Reference 03

Paznekas W.A., Genomics 62:42-49 (1999) .

Other Product Names

SNAH, SNAI, Sna, Snail

Tested Applications

WB, IF

Protein ID

18765741

Physical Properties

Liquid

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