WEE1 (phospho Ser642) Antibody

Product Specifications

Background

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

NCBI Gene ID

7465

Swiss Prot

P30291

Accession Number

NP_003381.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p) -L-T) derived from Human WEE1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

100 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

WEE1

NCBI Official Name

WEE1 G2 checkpoint kinase

NCBI Organism

Homo sapiens

Background Reference 01

Watanabe N., EMBO J. 14:1878-1891 (1995) .

Background Reference 02

Cichutek A., Cytogenet. Cell Genet. 93:277-283 (2001) .

Background Reference 03

Igarashi M., Nature 353:80-83 (1991)

Other Product Names

WEE1A, WEE1hu, kinase WEE1

Tested Applications

Protein ID

4507917

Physical Properties

Liquid

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