MDM2 (phospho Ser186/Ser188) Antibody

Product Specifications

Background

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

NCBI Gene ID

4193

Swiss Prot

Q00987

Accession Number

NP_001138811.1

Host

Rabbit

Reactivity

Human

Immunogen

MDM2 (phospho Ser186/Ser188) antibody was raised against a peptide sequence around phosphorylation site of Ser186/Ser188 (H-K-S (p) -D-S (p) -I-S) derived from Human MDM2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

55 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.

Symbol

MDM2

NCBI Official Name

MDM2 oncogene, E3 ubiquitin protein ligase

NCBI Organism

Homo sapiens

Background Reference 01

M Zhou, Blood, Mar 1995; 85: 1608 - 1614.

Background Reference 02

Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.

Background Reference 03

M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.

Other Product Names

Oncoprotein Mdm2, p53-binding protein Mdm2, Ubiquitin-protein ligase E3 Mdm2

Tested Applications

Protein ID

89993689

Physical Properties

Liquid

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