PAK1 (phospho Ser199) Antibody

Product Specifications

Background

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

NCBI Gene ID

5058

Swiss Prot

Q13153

Accession Number

NP_002567.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PAK1 (phospho Ser199) antibody was raised against a peptide sequence around phosphorylation site of Serine 199 (T-K-S (p) -V-I) derived from Human PAK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

61 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

PAK1

NCBI Official Name

P21 protein (Cdc42/Rac) -activated kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Brown J.L., Curr. Biol. 6:598-605 (1996) .

Background Reference 02

Sells M.A., Curr. Biol. 7:202-210 (1997) .

Background Reference 03

The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Other Product Names

ADRB2, PAK 1, P65-PAK, P68-PAK

Tested Applications

WB, IHC

Protein ID

42794769

Physical Properties

Liquid

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