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HnRPD (phospho Ser83) Antibody

Product Specifications

Background

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

NCBI Gene ID

3184

Swiss Prot

Q14103

Accession Number

NP_112738.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

HnRPD (phospho Ser83) antibody was raised against a peptide sequence around phosphorylation site of Serine 83 (N-S-S (p) -P-R) derived from Human hnRPD .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

38 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

HNRNPD

NCBI Official Name

Heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)

NCBI Organism

Homo sapiens

Background Reference 01

Kajita Y., J. Biol. Chem. 270:22167-22175 (1995) .

Background Reference 02

Dempsey L.A., Genomics 49:378-384 (1998) .

Background Reference 03

Tolnay M., Biochem. J. 338:417-425 (1999) .

Background Reference 04

Grosset C., Cell 103:29-40 (2000)

Other Product Names

ROD, AUF1, HNRPD

Tested Applications

WB, IHC, IF

Protein ID

14110420

Physical Properties

Liquid

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