EPHA 2/3/4 (phospho Tyr588/596) Antibody

Product Specifications

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

NCBI Gene ID

1969;2042;2043

Swiss Prot

P29317; P29320; P54764

Accession Number

NP_004422.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

EPHA 2/3/4 (phospho Tyr588/596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p) -V-R) / (R-T-Y (p) -V-D) derived from Human EPHA2/3/4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

130 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

EPHA2, EPHA3, EPHA4

NCBI Official Name

EPH receptor A2, EPH receptor A3, EPH receptor A4

NCBI Organism

Homo sapiens

Background Reference 01

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324 (1990) .

Background Reference 02

Zhang Y., Mol. Cell. Proteomics 4:1240-1250 (2005) .

Background Reference 03

Zhuang G., J. Biol. Chem. 282:2683-2694 (2007)

Other Product Names

ECK, MPK-5, SEK2, kinase EphA2

Tested Applications

WB, IF

Protein ID

32967311

Physical Properties

Liquid

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