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Cyclin H (phospho Thr315) Antibody

Product Specifications

Background

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

NCBI Gene ID

902

Swiss Prot

P51946

Accession Number

NP_001230.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Cyclin H (phospho Thr315) antibody was raised against a peptide sequence around phosphorylation site of threonine 315 (E-W-T (p) -D-D) derived from Human Cyclin H .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

36 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CCNH

NCBI Official Name

Cyclin H

NCBI Organism

Homo sapiens

Background Reference 01

Maekelae T.P., Nature 371:254-257 (1994) .

Background Reference 02

Fisher R.P., Cell 78:713-724 (1994) .

Background Reference 03

The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Other Product Names

Cyclin H, p34, p37, MO15-associated protein

Tested Applications

WB

Protein ID

4502623

Physical Properties

Liquid

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