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C/EBP-ε (phospho Thr74) Antibody

Product Specifications

Background

C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

NCBI Gene ID

1053

Swiss Prot

Q15744

Accession Number

NP_001796.2

Host

Rabbit

Reactivity

Human

Immunogen

C/EBP-ε (phospho Thr74) antibody was raised against a peptide sequence around phosphorylation site of threonine 174 (L-A-T (p) -A-A) derived from Human C/EBP-ε.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CEBPE

NCBI Official Name

CCAAT/enhancer binding protein (C/EBP), epsilon

NCBI Organism

Homo sapiens

Background Reference 01

Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.

Background Reference 02

Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.

Background Reference 03

Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.

Background Reference 04

Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

Other Product Names

CEBPE, C/EBP epsilon, CCAAT/enhancer binding protein epsilon

Tested Applications

WB

Protein ID

28872800

Physical Properties

Liquid

Frequently Asked Questions

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