BAD (phospho Ser91/128) Antibody

Product Specifications

Background

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

NCBI Gene ID

572

Swiss Prot

Q92934

Accession Number

NP_004313.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BAD (phospho Ser91/128) antibody was raised against a peptide sequence around phosphorylation site of Serine 91 (E-P-S (p) -P-F) derived from Human BAD or Serine 128 (E-P-S (p) -P-F) derived from Mouse BAD.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

22 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

BAD

NCBI Official Name

BCL2-associated agonist of cell death

NCBI Organism

Homo sapiens

Background Reference 01

Wang H.-G., Cell 87:629-638 (1996) .

Background Reference 02

Ottilie S., J. Biol. Chem. 272:30866-30872 (1997) .

Background Reference 03

The MGC Project Team, Genome Res. 14:2121-2127 (2004) .

Other Product Names

BAD, BBC6, BCL2L8, Bcl- XL/Bcl-2 associated death promoter

Tested Applications

WB, IHC

Protein ID

10835069

Physical Properties

Liquid

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