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B-RAF (phospho Thr599) Antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

NCBI Gene ID

673

Swiss Prot

P15056

Accession Number

NP_004324.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

B-RAF (phospho Thr599) antibody was raised against a peptide sequence around phosphorylation site of threonine 599 (L-A-T (p) -V-K) derived from Human B-RAF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

84 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

Symbol

BRAF

NCBI Official Name

V-raf murine sarcoma viral oncogene homolog B1

NCBI Organism

Homo sapiens

Background Reference 01

Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.

Background Reference 02

Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.

Background Reference 03

R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.

Background Reference 04

Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

Other Product Names

B-RAF, RAFB1, RMIL, BRAF1, c-RMIL

Tested Applications

IHC

Protein ID

50403720

Physical Properties

Liquid

Curated Selection

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