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Bloom Syndrome (phospho Thr99) Antibody

Product Specifications

Background

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE) .

NCBI Gene ID

641

Swiss Prot

P54132

Accession Number

NP_000048.1

Host

Rabbit

Reactivity

Human

Immunogen

Bloom Syndrome (phospho Thr99) antibody was raised against a peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p) -Q-R) derived from Human Bloom Syndrome.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

159 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

BLM

NCBI Official Name

Bloom syndrome, RecQ helicase-like

NCBI Organism

Homo sapiens

Background Reference 01

Ellis N.A., Cell 83:655-666 (1995) .

Background Reference 02

Karow J.K., J. Biol. Chem. 272:30611-30614 (1997) .

Background Reference 03

Pichierri P., EMBO J. 23:3154-3163 (2004) .

Other Product Names

RECQ2, RECQL3, type 2, EC 3.6.1, RecQ protein-like 3

Tested Applications

WB, IHC

Protein ID

1705486

Physical Properties

Liquid

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