Ras-GRF1 (phospho Ser916) Antibody

Product Specifications

Background

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors) . Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist) -induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

NCBI Gene ID

19417

Swiss Prot

Q13972

Accession Number

NP_002882.3

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Ras-GRF1 (phospho Ser916) antibody was raised against a peptide sequence around phosphorylation site of Serine 916 (N-E-G (p) -T P) derived from Human Ras-GRF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

145 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

Symbol

Rasgrf1

NCBI Official Name

RAS protein-specific guanine nucleotide-releasing factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.

Background Reference 02

Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.

Background Reference 03

Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.

Background Reference 04

Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

Other Product Names

CDC25, GNRP, GRF1, RASGRF1

Tested Applications

IHC

Protein ID

6755288

Physical Properties

Liquid

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