Pim-1 (phospho Tyr309) Antibody

Product Specifications

Background

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3) . Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.

NCBI Gene ID

5292

Swiss Prot

P06803

Accession Number

NP_001230115.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Pim-1 (phospho Tyr309) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 309 (A-A-V (p) -W-S) derived from Human Pim-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PIM1

NCBI Official Name

Pim-1 oncogene

NCBI Organism

Homo sapiens

Background Reference 01

Selten G., Cell 46:603-611 (1986) .

Background Reference 02

Saris C.J., EMBO J. 10:655-664 (1991) .

Background Reference 03

Maita H., Eur. J. Biochem. 267:5168-5178 (2000) .

Other Product Names

PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1

Tested Applications

Protein ID

83305339

Physical Properties

Liquid

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