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CD32 (phospho Tyr292) Antibody

Product Specifications

Background

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

NCBI Gene ID

2213

Swiss Prot

P31994

Accession Number

NP_003992.3

Host

Rabbit

Reactivity

Human

Immunogen

CD32 (phospho Tyr292) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 292 (I-T-Y (p) -S-L) derived from Human CD32.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

32 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

FCGR2B

NCBI Official Name

Fc fragment of IgG, low affinity IIb, receptor (CD32)

NCBI Organism

Homo sapiens

Background Reference 01

Stuart S.G., EMBO J. 8:3657-3666 (1989) .

Background Reference 02

Brooks D.G., J. Exp. Med. 170:1369-1385 (1989) .

Background Reference 03

Engelhardt W., Eur. J. Immunol. 20:1367-1377 (1990) .

Other Product Names

CD32, FCG2, FCG2B, , LY-17, CDW32

Tested Applications

WB

Protein ID

50511930

Physical Properties

Liquid

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