ACK1 (phospho Tyr284) Antibody

Product Specifications

Background

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.

NCBI Gene ID

10188

Swiss Prot

Q07912

Accession Number

NP_005772.3

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

ACK1 (phospho Tyr284) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y (p) -V-M) derived from Human ACK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

120 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

TNK2

NCBI Official Name

Tyrosine kinase, non-receptor, 2

NCBI Organism

Homo sapiens

Background Reference 01

Manser E., Nature 363:364-367 (1993) .

Background Reference 02

Ota T., Nat. Genet. 36:40-45 (2004) .

Background Reference 03

Eisenmann K.M., Nat. Cell Biol. 1:507-513 (1999)

Other Product Names

ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1

Tested Applications

WB, IHC, IF

Protein ID

229462980

Physical Properties

Liquid

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