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SF1 (phospho Ser82) Antibody

Product Specifications

Background

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

NCBI Gene ID

7536

Swiss Prot

Q15637

Accession Number

NP_004621.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

SF1 (phospho Ser82) antibody was raised against a peptide sequence around phosphorylation site of Serine 82 (S-P-S (p) -P-E) derived from Human SF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

SF1

NCBI Official Name

Splicing factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Susan Y. Park, Development, May 2005; 132: 2415 - 2423.

Background Reference 02

Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.

Background Reference 03

Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.

Background Reference 04

Philipp Gut, Development, Oct 2005; 132: 4611 - 4619.

Other Product Names

BBP, CW17, SF01, ZFM1, ZNF162

Tested Applications

WB, IHC

Protein ID

42544130

Physical Properties

Liquid

Curated Selection

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