What is the catalog number for PKA alpha/beta CAT (phospho Thr197) Antibody?

The catalog number for PKA alpha/beta CAT (phospho Thr197) Antibody is 79-792.

What sizes are available for PKA alpha/beta CAT (phospho Thr197) Antibody?

PKA alpha/beta CAT (phospho Thr197) Antibody is available in the following size(s): 0.1 mg.

Is PKA alpha/beta CAT (phospho Thr197) Antibody in stock?

PKA alpha/beta CAT (phospho Thr197) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

PKA alpha/beta CAT (phospho Thr197) Antibody

Product Specifications

Background

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

NCBI Gene ID

5566

Swiss Prot

P17612

Accession Number

NP_002721.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p) -T-L) derived from Human PKA alpha/beta CAT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PRKACA

NCBI Official Name

Protein kinase, cAMP-dependent, catalytic, alpha

NCBI Organism

Homo sapiens

Background Reference 01

Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.

Background Reference 02

W Li, Learn. Mem., Jan 1996; 2: 320 - 333.

Background Reference 03

Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.

Background Reference 04

Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

Other Product Names

PRKACA , PRKACB, PKACA

Tested Applications

WB, IHC, IF

Protein ID

4506055

Physical Properties

Liquid

Frequently Asked Questions

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