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Estrogen Receptor-alpha (phospho Tyr537) Antibody

Product Specifications

Background

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

NCBI Gene ID

2099

Swiss Prot

P03372

Accession Number

NP_000116.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Estrogen Receptor-alpha (phospho Tyr537) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 537 (P-L-Y (p) -D-L) derived from Human Estrogen Receptor-alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

66 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of ER-alpha only when phosphorylated at tyrosine 537.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

ESR1

NCBI Official Name

Estrogen receptor 1

NCBI Organism

Homo sapiens

Background Reference 01

Sehime Temel, Endocrinology, Oct 2002; 143: 3974.

Background Reference 02

Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005; 2005: 302.

Background Reference 03

Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004; 287: R661 - R669.

Background Reference 04

Marcello Maggiolini, Cancer Res., Oct 1999

Other Product Names

ER, ESR, ESR1, ESTR, NR3A1

Tested Applications

WB, IHC

Protein ID

62821794

Physical Properties

Liquid

Frequently Asked Questions

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