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Arrestin 1 (phospho Ser412) Antibody

Product Specifications

Background

Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

NCBI Gene ID

408

Swiss Prot

P49407

Accession Number

NP_004032.2

Host

Rabbit

Reactivity

Human

Immunogen

Arrestin 1 (phospho Ser412) antibody was raised against a peptide sequence around phosphorylation site of Serine 412 (T-G-S (p) -P-Q) derived from Human Arrestin 1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

50 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

ARRB1

NCBI Official Name

Arrestin, beta 1

NCBI Organism

Homo sapiens

Background Reference 01

Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.

Background Reference 02

D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.

Background Reference 03

Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.

Background Reference 04

Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.

Other Product Names

ARR1, arrestin 2, arrestin beta 1, beta-arrestin-1

Tested Applications

WB, IHC

Protein ID

10880136

Physical Properties

Liquid

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