Caspase 9 (phospho Thr125) Antibody

Product Specifications

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) . Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

NCBI Gene ID

842

Swiss Prot

P55211

Accession Number

NP_001220.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Caspase 9 (phospho Thr125) antibody was raised against a peptide sequence around phosphorylation site of threonine 125 (P-E-T (p) -P-R) derived from Human Caspase 9.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

47 kDa

Fragment

IgG

Specificity

The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R) .