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C/EBP-alpha (phospho Ser21) Antibody

Product Specifications

Background

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

NCBI Gene ID

1050

Swiss Prot

P49715

Accession Number

NP_004355.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C/EBP-alpha (phospho Ser21) antibody was raised against a peptide sequence around phosphorylation site of Serine 21 (L-Q-S (p) -P-P) derived from Human C/EBP-alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CEBPA

NCBI Official Name

CCAAT/enhancer binding protein (C/EBP), alpha

NCBI Organism

Homo sapiens

Background Reference 01

Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.

Background Reference 02

Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.

Background Reference 03

Raphaël Métivier, Mol. Endocrinol., Nov 2001; 15: 1953.

Background Reference 04

Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

Other Product Names

CAP4, ICE8, MACH, MCH5, Mch-5

Tested Applications

WB, IHC

Protein ID

28872794

Physical Properties

Liquid

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