BTK (phospho Tyr223) Antibody

Product Specifications

Background

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.

NCBI Gene ID

695

Swiss Prot

Q06187

Accession Number

NP_000052.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BTK (phospho Tyr223) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p) -D-Y) derived from Human BTK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

80 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

BTK

NCBI Official Name

Bruton agammaglobulinemia tyrosine kinase

NCBI Organism

Homo sapiens

Background Reference 01

Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676.

Background Reference 02

Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633.

Background Reference 03

Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449.

Background Reference 04

Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.

Other Product Names

AGMX1, ATK, kinase Btk, BPK

Tested Applications

Protein ID

547759

Physical Properties

Liquid

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