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Synaptotagmin (phospho Thr202) Antibody

Product Specifications

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.

NCBI Gene ID

6857

Swiss Prot

P21579

Accession Number

NP_001129277.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synaptotagmin (phospho Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (R-K-T (p) -L-N) derived from Human Synaptotagmin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

SYT1

NCBI Official Name

Synaptotagmin I

NCBI Organism

Homo sapiens

Background Reference 01

E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.

Background Reference 02

Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.

Background Reference 03

Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.

Background Reference 04

Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

Other Product Names

LAG, LAP18, STN1, Prosolin, stathmin

Tested Applications

WB, IHC

Protein ID

5032139

Physical Properties

Liquid

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