C Fos Antibody

Product Specifications

Background

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

NCBI Gene ID

2353

Swiss Prot

P01100

Accession Number

NP_005243.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C-Fos antibody was raised against a peptide sequence around aa.28~32 (S-Y-Y-H-S) derived from Human C-Fos.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

62 kDa

Specificity

This antibody detects endogenous level of total C-Fos protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

FOS

NCBI Official Name

FBJ murine osteosarcoma viral oncogene homolog

NCBI Organism

Homo sapiens

Background Reference 01

Okazaki K., Sagata N. EMBO J. 14:5048-5059 (1995) Zhang Y., Feng X. H., Derynck R. Nature 394:909-913 (1998) Bossis G., Malnou C. E., Farras R., Andermarcher E., Hipskind R., Rodriguez M., Schmidt D., Muller S., Jariel-Encontre I., Piechaczyk M. Mol. Cell. Biol. 25:6964-6979 (2005)