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PPARgamma Antibody

Product Specifications

Background

Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.

NCBI Gene ID

5468

Swiss Prot

P37231

Accession Number

NP_005028.4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

PPARgamma antibody was raised against a peptide sequence around aa.50~54 (L-S-V-M-E) derived from Human PPARγ .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-749

Calculated Molecular Weight

53 57 kDa

Specificity

This antibody detects endogenous levels of total PPARγ protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PPARG

NCBI Official Name

Peroxisome proliferator-activated receptor gamma

NCBI Organism

Homo sapiens

Background Reference 01

"Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARgamma2 versus PPARgamma1 and activation with retinoid X receptor agonists and antagonists. "Mukherjee R., Jow L., Croston G. E., et al. J. Biol. Chem. 272:8071-8076 (1997) "Cloning and characterization of RAP250, a nuclear receptor coactivator. "Caira F., Antonson P., Pelto-Huikko M., et al. J. Biol. Chem. 275:5308-5317 (2000) "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha. "Qi C., Chang J., Zhu Y., et al. J. Biol. Chem. 277:28624-28630 (2002)

Other Product Names

GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma, MAPTL, MTBT1, TAU, GLM1, PPARG

Tested Applications

WB

Protein ID

116284368

Physical Properties

Liquid

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