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E2F 1 Antibody

Product Specifications

Background

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

NCBI Gene ID

1869

Swiss Prot

Q01094

Accession Number

NP_005216.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

E2F-1 antibody was raised against a peptide sequence around aa.430~434 (G-D-L-T-P) derived from Human E2F-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Apoptosis

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

70 kDa

Specificity

This antibody detects endogenous levels of total E2F-1 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

E2F1

NCBI Official Name

E2F transcription factor 1

NCBI Organism

Homo sapiens

Other Product Names

RBP3, E2F-1, RBAP1, RBBP3, RAF, RBP3

Tested Applications

WB

Protein ID

12669911

Physical Properties

Liquid

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