TAZ Antibody

Product Specifications

Background

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

NCBI Gene ID

25937

Swiss Prot

Q9GZV5

Accession Number

NP_001161750.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

TAZ antibody was raised against a peptide sequence around aa.386~390 (V-E-S-A-L) derived from Human TAZ.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

49 kDa

Specificity

This antibody detects endogenous level of total TAZ protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

WWTR1

NCBI Official Name

WW domain containing transcription regulator 1

NCBI Organism

Homo sapiens

Background Reference 01

Kanai F., Marignani P. A., Sarbassova D., Yagi R., Hall R. A., Donowitz M., Hisaminato A., Fujiwara T., Ito Y., Cantley L. C., Yaffe M. B. EMBO J. 19:6778-6791 (2000) Lei Q. Y., Zhang H., Zhao B., Zha Z. Y., Bai F., Pei X. H., Zhao S., Xiong Y., Guan K. L. Mol. Cell. Biol. 28:2426-2436 (2008) Varelas X., Sakuma R., Samavarchi-Tehrani P., Peerani R., Rao B. M., Dembowy J., Yaffe M. B., Zandstra P. W., Wrana J. L. Nat. Cell Biol. 10:837-848 (2008) Di Palma T., D'Andrea B., Liguori G. L., Liguoro A., de Cristofaro T., Del Prete D., Pappalardo A., Mascia A., Zannini M. Exp. Cell Res. 315:162-175 (2009)