GAPDH Antibody

Product Specifications

Background

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

NCBI Gene ID

2597

Swiss Prot

P04406

Accession Number

NP_002037.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

GAPDH antibody was raised against a peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

37 kDa

Specificity

This antibody detects endogenous level of total GAPDH protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

GAPDH

NCBI Official Name

Glyceraldehyde-3-phosphate dehydrogenase

NCBI Organism

Homo sapiens

Background Reference 01

Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341 (1988) Tisdale E. J. J. Biol. Chem. 277:3334-3341 (2002)

Other Product Names

G3PD, GAPD, HEL-S-162eP, G3PD, MGC88685

Tested Applications

Protein ID

7669492

Physical Properties

Liquid

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