CD209 (DC SIGN) Antibody

Product Specifications

Background

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

NCBI Gene ID

30835

Swiss Prot

Q9NNX6

Accession Number

NP_066978.1

Host

Rabbit

Reactivity

Human

Immunogen

CD209 (DC-SIGN) antibody was raised against a peptide sequence around aa.389~393 (E-Q-F-L-S) derived from Human CD209 (DC-SIGN) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

46 kDa

Specificity

This antibody detects endogenous level of total CD209 (DC-SIGN) protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CD209

NCBI Official Name

CD209 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Geijtenbeek T. B. H., Kwon D. S., Torensma R., van Vliet S. J., van Duijnhoven G. C. F., Middel J., Cell 100:587-597 (2000) Geijtenbeek T. B. H., Krooshoop D. J., Bleijs D. A., van Vliet S. J., van Duijnhoven G. C. F., Grabovsky Nat. Immunol. 1:353-357 (2000) Engering A., Geijtenbeek T. B. H., van Vliet S. J., van Kooyk Y. J. Immunol. 168:2118-2126 (2002)