HnRNP A1 (Ab 192) Antibody

Product Specifications

Background

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism and transport of nuclear pre-mRNA transcripts . hnRNP A1 regulates the alternative splicing of c-Src, c-H-Ras and modifies initiation of translation of the fibroblast growth factor 2 mRNA. hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells . Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus . hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production . HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm

NCBI Gene ID

3178

Swiss Prot

P09651

Accession Number

NP_002127.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HnRNP A1 (Ab-192) antibody was raised against a peptide sequence around aa.190~194 (S-S-S-Q-R) derived from Human hnRNP A1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34, 39 kDa

Specificity

This antibody detects endogenous levels of total hnRNP A1 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

HNRNPA1

NCBI Official Name

Heterogeneous nuclear ribonucleoprotein A1

NCBI Organism

Homo sapiens

Background Reference 01

Myer, V. E. and Steitz, J. A. (1995) RNA 1, 171-82. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53. Patry, C. et al. (2003) Cancer Res 63, 7679-88. Pinol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2. Siomi, M. C. et al. (1997) J Cell Biol 138, 1181-92. Black, A. C. et al. (1996) Virus Genes 12, 275-85. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91. Monette, A. et al. (2009) J Biol Chem 284, 31350-62.