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Vimentin Antibody

Product Specifications

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

NCBI Gene ID

7431

Swiss Prot

P08670

Accession Number

NP_003371.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Vimentin antibody was raised against a peptide sequence around aa.452~456 (G-Q-V-I-N) derived from Human Vimentin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

57 kDa

Specificity

This antibody detects endogenous level of total Vimentin protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

VIM

NCBI Official Name

Vimentin

NCBI Organism

Homo sapiens

Background Reference 01

Sommers C. L., Walker-Jones D., Heckford S. E., Worland P., Valverius E., Clark R., McCormick F., Cancer Res. 49:4258-4263 (1989) Kang S. -M., Shin M. -J., Kim J. -H., Oh J. -W. Proteomics 5:2227-2237 (2005) Ahmed B. A., Bukhari I. A., Jeffus B. C., Harney J. T., Thyparambil S., Ziu E., Fraer M., Rusch N. J., PLoS ONE 4:E4730-E4730 (2009)

Other Product Names

HEL113, CTRCT30, FLJ36605, VIM

Tested Applications

WB

Protein ID

62414289

Physical Properties

Liquid

Frequently Asked Questions

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