E Cadherin (CD324) Antibody

Product Specifications

Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

NCBI Gene ID

999

Swiss Prot

P12830

Accession Number

NP_004351.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E-Cadherin (CD324) antibody was raised against a peptide sequence around aa.864~868 (E-W-G-N-R) derived from Human E-Cadherin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

135 kDa

Specificity

This antibody detects endogenous level of total E-Cadherin protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CDH1

NCBI Official Name

Cadherin 1, type 1, E-cadherin (epithelial)

NCBI Organism

Homo sapiens

Background Reference 01

Baki L. et. al (2001) Proc. Natl. Acad. Sci. U. S. A. 98:2381-2386Makagiansar I. T. et. al (2002) J. Biol. Chem. 277:16002-16010Agiostratidou G. (2006) J. Neurochem. 96:1182-1188