CD56 (NCAM) Antibody

Product Specifications

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1) . NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2) . CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3) .

NCBI Gene ID

4684

Swiss Prot

P13591

Accession Number

NP_000606.3

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

CD56 (NCAM) antibody was raised against a peptide sequence around aa.850~854 (Q-T-K-E-N) derived from Human CD56 (NCAM) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

120 - 220 kDa

Specificity

This antibody detects endogenous levels of total NCAM protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

NCAM1

NCBI Official Name

Neural cell adhesion molecule 1

NCBI Organism

Homo sapiens

Background Reference 01

Cunningham, B. A. et al. (1987) Science 236, 799-806. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918. Robertson, M. J. and Ritz, J. (1990) Blood 76, 2421-2438.