MEK1 / 2 Antibody

Product Specifications

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

NCBI Gene ID

5604

Swiss Prot

Q02750

Accession Number

NP_002746.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK1/2 antibody was raised against a peptide sequence around aa.235-239 (L-Q-G-T-H) derived from Human MEK1/2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Specificity

This antibody detects endogenous level of total MEK1/2 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MAP2K1

NCBI Official Name

Mitogen-activated protein kinase kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Seger R., Seger D., Lozeman F. J., Ahn N. G., Graves L. M., Campbell J. S., Ericsson L., Harrylock M., Jensen A. M., Krebs E. G. J. Biol. Chem. 267:25628-25631 (1992) Mukherjee S., Keitany G., Li Y., Wang Y., Ball H. L., Goldsmith E. J., Orth K. Science 312:1211-1214 (2006)