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Caspase 9 Antibody

Product Specifications

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) . Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

NCBI Gene ID

842

Swiss Prot

P55211

Accession Number

NP_001220.2

Host

Rabbit

Reactivity

Human

Immunogen

Caspase 9 antibody was raised against a peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

47, 37, 35 kDa

Specificity

This antibody detects endogenous level of total Caspase 9 protein.

Applications Notes

Western Blot: 1:1000

Symbol

CASP9

NCBI Official Name

Caspase 9, apoptosis-related cysteine peptidase

NCBI Organism

This antibody was raised against a peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9

Background Reference 01

Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. Li, P. et al. (1997) Cell 91, 479-489.

Other Product Names

MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, ICE-like apoptotic protease 6

Tested Applications

WB

Protein ID

14790124

Physical Properties

Liquid

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