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Caspase 3 Antibody

Product Specifications

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

NCBI Gene ID

836

Swiss Prot

P42574

Accession Number

NP_004337.2

Host

Rabbit

Reactivity

Human

Immunogen

Caspase 3 antibody was raised against a peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

35 19 17 kDa

Specificity

This antibody detects endogenous level of total Caspase 3 protein.

Applications Notes

Western Blot: 1:1000

Symbol

CASP3

NCBI Official Name

Caspase 3, apoptosis-related cysteine peptidase

NCBI Organism

This antibody was raised against a peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.

Background Reference 01

Gervais, F. G et al. Cell 97: 395-406, 1999. Nicholson, D. W. et al. (1995) Nature 376, 37-43.

Other Product Names

CPP32, SCA-1, CPP32B, SCFR, Apopain

Tested Applications

WB

Protein ID

14790119

Physical Properties

Liquid

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