LC3 Antibody

Product Specifications

Background

Probably involved in formation of autophagosomal vacuoles (autophagosomes) .

NCBI Gene ID

84557

Swiss Prot

Q9H492

Accession Number

NP_115903.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

LC3 antibody was raised against a peptide sequence around aa. 112-116 (V-Y-A-S-Q) derived from Human LC3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

19 kDa

Specificity

This antibody detects endogenous level of total LC3 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MAP1LC3A

NCBI Official Name

Microtubule-associated protein 1 light chain 3 alpha

NCBI Organism

Homo sapiens

Background Reference 01

He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J. O., Yu L. J. Biol. Chem. 278:29278-29287 (2003) Tanida I., Sou Y. -S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276 (2004)