CARM1 (Ab 228) Antibody

Product Specifications

Background

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-κ -B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

NCBI Gene ID

10498

Swiss Prot

Q86X55

Accession Number

NP_954592.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CARM1 (Ab-228) antibody was raised against a peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

63 kDa

Specificity

This antibody detects endogenous levels of total CARM1 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

CARM1

NCBI Official Name

Coactivator-associated arginine methyltransferase 1

NCBI Organism

This antibody was raised against a peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1

Background Reference 01

Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103 (36) : 13351–13356. Marcela Covic, et al. (2005) EMBO J; 24 (1) : 85–96. Eleni Zika et al. (2005) Proc Natl Acad Sci U S A. ; 102 (45) : 16321–16326Neelu Yadav et al. (2003) Proc Natl Acad Sci U S A; 100 (11) : 6464–6468