VASP (Ab 157) Antibody
Product Specifications
Background
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
NCBI Gene ID
7408
Swiss Prot
P50552
Accession Number
NP_003361.1
Host
Rabbit
Reactivity
Human, Rat
Immunogen
VASP (Ab-157) antibody was raised against a peptide sequence around aa.155~159 (R-V-S-N-A) derived from Human VASP.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
50 kDa
Specificity
This antibody detects endogenous level of total VASP protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Symbol
VASP
NCBI Official Name
Vasodilator-stimulated phosphoprotein
NCBI Organism
Homo sapiens
Background Reference 01
Zhao WM, et al. (2001) EMBO J 20 (9) : 2315-2325. Millard TH, et al. (2005) EMBO J 24 (2) : 240-250. Kühnel K, et al. (2004) Proc Natl Acad Sci U S A 101 (49) : 17027-17032. Haffner C, et al. (1995) EMBO J 14 (1) : 19-27. Ramesh N, et al. (1997) Proc Natl Acad Sci U S A 94 (26) : 14671-14676.
Other Product Names
VASP
Tested Applications
WB, IF
Protein ID
4507869
Physical Properties
Liquid
Frequently Asked Questions
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