FLT3 (Ab 591) Antibody

Product Specifications

Background

FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

NCBI Gene ID

2322

Swiss Prot

P36888

Accession Number

NP_004110.2

Host

Rabbit

Reactivity

Human

Immunogen

FLT3 (Ab-591) antibody was raised against a peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

130, 160 kDa

Specificity

This antibody detects endogenous level of total FLT3 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

FLT3

NCBI Official Name

Fms-related tyrosine kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Sekine S et, al. (2008) J Immunol. ;180 (12) : 8126-34Pratz K et, al. (2008) Leuk Lymphoma. 2008 ;49 (5) : 852-63. Al Shaer L et, al. (2008) Br J Haematol. 141 (4) : 483-93.